澳门皇冠体育

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冰冻切片线粒体内膜功能(膜电位)荧光测定试剂盒产品专业指导
点击次数:142 发布时间:2019-11-04
 

澳门皇冠体育HL10013.6 v.A

 

BINGDONGQIEPIANXIANLITINEIMOGONGNENG(MODIANWEI)YINGGUANGCEDINGSHIJIHECHANPINSHUOMINGSHU

 

ZHUYAOYONGTU

 

BINGDONGQIEPIANXIANLITINEIMOGONGNENG(MODIANWEI)YINGGUANGCEDINGSHIJISHIYIZHONGZHIZAITONGGUOGAODUMINGANDEYANGLIZIYINGGUANGTANGHUAQINGRANLIAOJIEHEDAOXIANLITIJIZHI,QIYINGGUANGDEZENGQIANGHUOJIANRUOSHUOMINGXIANLITIMODIANWEIDEBIANHUA,JINEIMODIANFUXINGDEZENGGAOHUOJIANGDI,LAIFENXIBINGDONGZUZHIYANGPINXIANLITINEIMOGONGNENGDEWANZHENGXINGDEERJINGDIANDEJISHUFANGFA。GAIJISHUJINGGUOJINGXINYANZHI、CHENGGONGSHIYANZHENGMINGDE。QISHIYONGYUGEZHONGBINGDONGDONGWUZUZHIXIANLITIDEGONGNENGJIANCE。KEYIBEIYONGYUXIBAODIAOWANG、XINHAOCHUANDI、SHUAILAODENGDEYANJIU。CHANPINYANGEWUJUN,JIDAOJIYONG,HUOTIJIANCE,CAOZUOJIWEIJIANYI,XINGNENGWENDING。

 

JISHUBEIJING

 

YANGLIZIYINGGUANGTANGHUAQINGRANSEJIJC-1(5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide)SHIYIZHONGDUIMODIANWEIGAODUMINGANDERANSEJI。TATEYIXINGDIJINRUXIANLITI,FENBUHEJIEHEZAIXIANLITIJIZHISHANG。XIANLITIMODIANWEIDEGAODIBIANHUAJUEDINGLEJC-1DEFENBUNONGDU。DIANWEIGAO,JC-1XINGCHENGJUJITI,ERNONGDUGAO,YINGGUANGXIANSEWEIHONGSEHUOJIEHONGSE;FANZHI,ZEWEILVSE。YINGGUANGJIANRUOBIAOMINGXIANLITINEIMOGONGNENGSHOUDAOSUNHAI。 

 

CHANPINNEIRONG

 

RANSEYE(Reagent A)     40WEISHENG

XISHIYE(Reagent B)      5HAOSHENG

澳门皇冠体育QINGLIYE(Reagent C)      20HAOSHENG

澳门皇冠体育CHANPINSHUOMINGSHU       1FEN

 

BAOCUNFANGSHI

 

BAOCUNQINGLIYE(Reagent C)ZAI4℃BINGXIANGLI,QIYUDEBAOCUNZAI-20℃BINGXIANGLI;RANSEYE(Reagent A)BIMIANGUANGZHAO;YOUXIAOBAOZHENG6YUE

 

YONGHUZIBEI

 

澳门皇冠体育1.5HAOSHENGLIXINGUAN:YONGYUGONGZUOYEPEIZHIDERONGQI

PEIYANGXIANG:YONGYURANSEFUYU

(GONGJUJIAO)YINGGUANGXIANWEIJING:YONGYUBINGDONGQIEPIANZUZHIXIBAOXIANLITIYINGGUANGDINGXINGFENXI

 

SHIYANBUZHOU

 

SHIYANKAISHIQIAN,JIANG-20℃BINGXIANGLIDESHIJIHEZHONGDERANSEYE(Reagent A)ZHIRUBINGCAOLIRONGHUA,XISHIYE(Reagent B)FANGJIN37℃HENGWENSHUICAOLIYURE。RANHOUYICHU10WEISHENGRANSEYE(Reagent A)DAOXINDE1.5HAOSHENGLIXINGUAN,JIARU890WEISHENGXISHIYE(Reagent B),HUNYUNHOU,ZAIBINGCAOLIJINGZHI,BINGBIAOJIWEIRANSEGONGZUOYE,FANGZAIANSHILI。RANHOUJINXINGXIALIECAOZUO。

 

1.ZHUNBEI5PIANDAICEDEHOUWEI10WEIMIDEWEIJINGGUZHECHULIDEBINGDONGQIEPIAN

2.ZHIYUSHIWENXIA,XIAOXINJIASHANG500WEISHENGYULENGDEQINGLIYE( Reagent C),PUMANZHENGGEQIEPIANBIAOMIAN

3.XIAOXINYIQUQIEPIANSHANGDEQINGLIYE(Reagent C)

4.XIAOXINJIASHANG200WEISHENGSHIWENYUREDERANSEGONGZUOYE,PUMANZHENGGEQIEPIANBIAOMIAN

5.ZAI37℃SHIRUNPEIYANGXIANGLI,FUYU20FENZHONG(ZHUYI:BIMIANYETIZHENGFA)

澳门皇冠体育6.XIAOXINYIQUQIEPIANSHANGDERANSEGONGZUOYE,BIMIANGUANGZHAO

7.XIAOXINJIASHANG500WEISHENGQINGLIYE(Reagent C),PUMANZHENGGEQIEPIANBIAOMIAN

8.XIAOXINYIQUQIEPIANSHANGDEQINGLIYE(Reagent C)

9.FANGSHANGGAIBOPIANHUOFENGPIAN(ZHUYI:JIANCEQIANZHIYUBINGCAOLI)

10.JIKEZAIDAOZHIYINGGUANGXIANWEIJINGXIAJINXINGGUANCHA(DANLVBOHUOSHUANGLVBO):

 

澳门皇冠体育GUANCHAHONGSEYINGGUANG:LVBOQIJIFABOZHANG490nm,SANFABOZHANG590nmHUOLUODANMING(rhodamine)LVBOQIJIFABOZHANG540nm,

              SANFABOZHANG570nmHUODEZHOUHONG(Texas Red)LVBOQIJIFABOZHANG590nm,SANFABOZHANG610nm--KEJIAN

澳门皇冠体育              LIANGHONGSEYINGGUANG;RUGUOQIANGDUXIANZHUJIANRUO,BIAOMINGXIANLITIMODIANWEISHOUDAOPOHUAI

 

GUANCHALVSEYINGGUANG:LVBOQIJIFABOZHANG490nm,SANFABOZHANG530nmHUOYINGGUANGSU(Fluorescein)LVBOQIJIFABOZHANG490nm,

澳门皇冠体育              SANFABOZHANG520nm--RUGUOLVSEYINGGUANGQIANGDUXIANZHUZENGQIANG,BIAOMINGXIANLITIMODIANWEISHOUDAOPOHUAI,WEIJIEHEJC

              -1RANLIAOZAIXIBAOJIANGLI

ZHUYISHIXIANG

 

澳门皇冠体育1.BENCHANPINWEI20CICAOZUO

2.CAOZUOSHI,XUDAISHOUTAO

澳门皇冠体育3.RANSEGONGZUOYEBIMIANGUANGZHAO

澳门皇冠体育4.YONGHUGENJUSHIJIXUQIU,ANBILIPEIZHISHIJIYONGLIANG

澳门皇冠体育5.GENJUBUTONGZUZHILEIXING,DIAOZHENGRANSEGONGZUOYEDESHIYONGJILIANG(1:100ZHI1:1000RONGLIANGBI),BIMIANGUODURANSE

6.JIANYIQIEPIANZUZHIRANSEWANCHENGHOU,JIKEJINXINGYINGGUANGJIANCEFENXI

7.FUYUSHI,BIMIANGUANGZHAO

澳门皇冠体育8.SHUANGLVBOHUOSHUANGZHONGCEDING:JIANKANGXIBAOHEXIANLITICHENGXIANHONGSE;SIWANGHUODIAOWANGXIBAOJISUNSHANGXIANLITICHENGXIANLVSE

9.BENGONGSITIGONGXILIEXIANLITISHIJICHANPIN

 

ZHILIANGBIAOZHUN

 

澳门皇冠体育1.BENCHANPINJINGJIANDINGXINGNENGWENDING

澳门皇冠体育2.BENCHANPINJINGJIANDINGYINGGUANGQINGXI